Human IgG4 monoclonal antibody, clone RM120
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More Files
- More Functions
Western Blot
Western blot analysis of human, mouse, rat, and goat IgG using Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) under 0.5 ug/mL working concentration.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) under 5 ug/mL working concentration.
Immunohistochemistry
Immunohistochemical staining of FFPE Tonsil tissue sections using Human IgG4 monoclonal antibody, clone RM120 (Cat# MAB12821) at a 1:50,000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 monoclonal antibody, clone RM120 (Cat# MAB12821) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
ELISA showing Human IgG4 monoclonal antibody, clone RM120 (Cat# MAB12821) does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Cat# MAB12821 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Enzyme-linked Immunoabsorbent Assay
Detection of human IgG4 in monkey serum. Sandwich ELISA using Human IgG4 monoclonal antibody, clone RM120 (Cat# MAB12821) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by a HRP conjugated streptavidin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human IgG4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to the hinge region of human IgG4.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
Flow Cytometry
Immunocytochemistry (1-10 ug/mL)
Immunohistochemistry (1-10 ug/mL)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of human, mouse, rat, and goat IgG using Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) under 0.5 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) under 5 ug/mL working concentration.Immunohistochemistry
Immunohistochemical staining of FFPE Tonsil tissue sections using Human IgG4 monoclonal antibody, clone RM120 (Cat# MAB12821) at a 1:50,000 dilution.Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG4 monoclonal antibody, clone RM120 (Cat # MAB12821) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgG4 monoclonal antibody, clone RM120 (Cat# MAB12821) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an AP conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
ELISA showing Human IgG4 monoclonal antibody, clone RM120 (Cat# MAB12821) does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Cat# MAB12821 and a monkey IgG binding antibody (positive control) was used as the detection antibody.Enzyme-linked Immunoabsorbent Assay
Detection of human IgG4 in monkey serum. Sandwich ELISA using Human IgG4 monoclonal antibody, clone RM120 (Cat# MAB12821) as the capture antibody, and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by a HRP conjugated streptavidin.Flow Cytometry
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Gene Info — IGHG4
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Interactome
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Publication Reference
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Epstein-Barr Virus Lytic Reactivation Induces IgG4 Production by Host B Lymphocytes in Graves' Disease Patients and Controls: A Subset of Graves' Disease Is an IgG4-Related Disease-Like Condition.
Nagata K, Hara S, Nakayama Y, Higaki K, Sugihara H, Kuwamoto S, Matsushita M, Kato M, Tanio S, Ishiguro K, Hayashi K.
Viral Immunology 2018 Oct; 31(8):540.
Application：IF, IHC, Human, PBMCs, Thyroid gland.
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Epstein-Barr Virus Lytic Reactivation Induces IgG4 Production by Host B Lymphocytes in Graves' Disease Patients and Controls: A Subset of Graves' Disease Is an IgG4-Related Disease-Like Condition.
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