TUBE1 monoclonal antibody, clone 7G3B2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [TUBE1 (AA: 314-472)-hIgGFc transfected HEK293 cells] with TUBE1 monoclonal antibody, clone 7G3B2 (Cat# MAB12240) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human TUBE1 (AA: 314-472) recombinant protein (Expected MW is 44.3 kDa) with TUBE1 monoclonal antibody, clone 7G3B2 (Cat# MAB12240) at 1:500-1:2000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TUBE1 monoclonal antibody, clone 7G3B2 (Cat# MAB12240) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells with TUBE1 monoclonal antibody, clone 7G3B2 (Cat# MAB12240) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human TUBE1.
Immunogen
Recombinant protein corresponding to amino acids 314-472 of human TUBE1.
Host
Mouse
Theoretical MW (kDa)
53
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [TUBE1 (AA: 314-472)-hIgGFc transfected HEK293 cells] with TUBE1 monoclonal antibody, clone 7G3B2 (Cat# MAB12240) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human TUBE1 (AA: 314-472) recombinant protein (Expected MW is 44.3 kDa) with TUBE1 monoclonal antibody, clone 7G3B2 (Cat# MAB12240) at 1:500-1:2000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TUBE1 monoclonal antibody, clone 7G3B2 (Cat# MAB12240) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of Hela cells with TUBE1 monoclonal antibody, clone 7G3B2 (Cat# MAB12240) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — TUBE1
Entrez GeneID
51175Gene Name
TUBE1
Gene Alias
FLJ22589, TUBE, dJ142L7.2
Gene Description
tubulin, epsilon 1
Omim ID
607345Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the tubulin superfamily. This protein localizes to the centriolar sub-distal appendages that are associated with the older of the two centrioles after centrosome duplication. This protein plays a central role in organization of the microtubules during centriole duplication. A pseudogene of this gene is found on chromosome 5
Other Designations
OTTHUMP00000017033|OTTHUMP00000040425|epsilon-tubulin
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Interactome
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