Acidic Cytokeratin monoclonal antibody, clone SPM115
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of VIM in A-431 cell lysate with Acidic Cytokeratin monoclonal antibody, clone SPM115 (Cat# MAB12076).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with Acidic Cytokeratin monoclonal antibody, clone SPM115 (Cat# MAB12076).
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Specification
Product Description
Mouse monoclonal antibody raised against Acidic Cytokeratin.
Immunogen
Solubilized keratin extract from human stratum corneum.
Host
Mouse
Theoretical MW (kDa)
40-57
Reactivity
Human, Mouse, Rabbit, Rat
Specificity
This monoclonal antibody recognizes KRT10 (56.5kDa), KRT14 (50kDa), KRT15 (50kDa), KRT16 (48kDa), and KRT19 (40kDa).
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Immunohistochemistry (0.5-1 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of VIM in A-431 cell lysate with Acidic Cytokeratin monoclonal antibody, clone SPM115 (Cat# MAB12076).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with Acidic Cytokeratin monoclonal antibody, clone SPM115 (Cat# MAB12076). -
Gene Info — KRT10
Entrez GeneID
3858Gene Name
KRT10
Gene Alias
CK10, K10, KPP
Gene Description
keratin 10
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq
Other Designations
cytokeratin 10
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Gene Info — KRT14
Entrez GeneID
3861Gene Name
KRT14
Gene Alias
CK14, EBS3, EBS4, K14, NFJ
Gene Description
keratin 14
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq
Other Designations
cytokeratin 14|keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
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Gene Info — KRT15
Entrez GeneID
3866Gene Name
KRT15
Gene Alias
CK15, K15, K1CO
Gene Description
keratin 15
Omim ID
148030Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq
Other Designations
OTTHUMP00000165060|cytokeratin 15|keratin-15, basic|keratin-15, beta|type I cytoskeletal 15
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Gene Info — KRT16
Entrez GeneID
3868Gene Name
KRT16
Gene Alias
CK16, K16, K1CP, KRT16A, NEPPK
Gene Description
keratin 16
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq
Other Designations
cytokeratin 16|focal non-epidermolytic palmoplantar keratoderma|keratin, type I cytoskeletal 16
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Gene Info — KRT19
Entrez GeneID
3880Gene Name
KRT19
Gene Alias
CK19, K19, K1CS, MGC15366
Gene Description
keratin 19
Omim ID
148020Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations
40-kDa keratin intermediate filament|cytokeratin 19|keratin, type I cytoskeletal 19|keratin, type I, 40-kd
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