Pan Cytokeratin monoclonal antibody, clone AE1 + AE3
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon carcinoma with Pan Cytokeratin monoclonal antibody, clone AE1 + AE3 (Cat # MAB11311).
Flow Cytometry
Flow cytometric analysis of human Pan-Cytokeratins on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Pan Cytokeratin monoclonal antibody, clone AE1 + AE3.
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Specification
Product Description
Mouse monoclonal antibody raised against Pan Cytokeratin.
Immunogen
Human epidermal keratin.
Host
Mouse
Theoretical MW (kDa)
40-67
Reactivity
Human
Specificity
This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8); 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19). AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1-2 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Western Blot (1-2 ug/mL for 2 hours at RT)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon carcinoma with Pan Cytokeratin monoclonal antibody, clone AE1 + AE3 (Cat # MAB11311).Immunofluorescence
Flow Cytometry
Flow cytometric analysis of human Pan-Cytokeratins on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Pan Cytokeratin monoclonal antibody, clone AE1 + AE3. -
Gene Info — KRT76
Entrez GeneID
51350Gene Name
KRT76
Gene Alias
HUMCYT2A, KRT2B, KRT2P
Gene Description
keratin 76
Gene Ontology
HyperlinkGene Summary
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq
Other Designations
cytokeratin 2|keratin 2p
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Gene Info — KRT77
Entrez GeneID
374454Gene Name
KRT77
Gene Alias
K1B, KRT1B, MGC148087
Gene Description
keratin 77
Omim ID
611158Gene Ontology
HyperlinkGene Summary
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13
Other Designations
keratin 1B
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