KRT19 monoclonal antibody, clone A53-B/A2.26
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HepG2 and MCF7 cell lysates using KRT19 monoclonal antibody, clone A53-B/A2.26 (Cat # MAB11278) at 1 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with KRT19 monoclonal antibody, clone A53-B/A2.26 (Cat # MAB11278) at 1:100 using peroxidase-conjugate and DAB chromogen.
Immunofluorescence
Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with KRT19 monoclonal antibody, clone A53-B/A2.26 (Cat # MAB11278) followed by Goat anti-mouse IgG-CF488 (Cyan). The nuclear counterstain is Reddot (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against KRT19.
Immunogen
Human breast cancer MCF-7 cells.
Host
Mouse
Theoretical MW (kDa)
40
Reactivity
Human
Specificity
This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2a, lambda
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 and MCF7 cell lysates using KRT19 monoclonal antibody, clone A53-B/A2.26 (Cat # MAB11278) at 1 ug/ml.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with KRT19 monoclonal antibody, clone A53-B/A2.26 (Cat # MAB11278) at 1:100 using peroxidase-conjugate and DAB chromogen.Immunofluorescence
Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with KRT19 monoclonal antibody, clone A53-B/A2.26 (Cat # MAB11278) followed by Goat anti-mouse IgG-CF488 (Cyan). The nuclear counterstain is Reddot (Red).Flow Cytometry
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Gene Info — KRT19
Entrez GeneID
3880Protein Accession#
P08727Gene Name
KRT19
Gene Alias
CK19, K19, K1CS, MGC15366
Gene Description
keratin 19
Omim ID
148020Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations
40-kDa keratin intermediate filament|cytokeratin 19|keratin, type I cytoskeletal 19|keratin, type I, 40-kd
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Interactome
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Disease
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