CSTB monoclonal antibody, clone 2F1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell lysate, Lane 2: U87MG cell lysate.
Immunofluorescence
Immunofluorescence analysis of A549 cells. The cell was stained with CSTB monoclonal antibody, clone 2F1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant CSTB.
Immunogen
Recombinant protein corresponding to full length human CSTB.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell lysate, Lane 2: U87MG cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of A549 cells. The cell was stained with CSTB monoclonal antibody, clone 2F1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CSTB
Entrez GeneID
1476GeneBank Accession#
NM_000100Protein Accession#
NP_000091Gene Name
CSTB
Gene Alias
CST6, EPM1, PME, STFB
Gene Description
cystatin B (stefin B)
Gene Ontology
HyperlinkGene Summary
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq
Other Designations
CPI-B|cystatin B|liver thiol proteinase inhibitor|stefin B
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Interactome
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Disease
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