GIPC2 monoclonal antibody, clone AT1G10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of recombinant human GIPC1, 2 (each 20 ng) and cell lysates (40 ug) by using GIPC2 monoclonal antibody, clone AT1G10 (Cat # MAB11190) (1:1000). Lane1 : GIPC1. Lane2 : GIPC2. Lane 3 : 293T. Lane 4 : HepG2. Lane 5 : HeLa.
Flow Cytometry
Flow cytometry analysis of GIPC in HeLa cells, The cell was stained with GIPC2 monoclonal antibody, clone AT1G10 (Cat # MAB11190) at 2-5 ug for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GIPC2.
Immunogen
Recombinant protein corresponding to amino acids 1-315 of human GIPC2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of recombinant human GIPC1, 2 (each 20 ng) and cell lysates (40 ug) by using GIPC2 monoclonal antibody, clone AT1G10 (Cat # MAB11190) (1:1000). Lane1 : GIPC1. Lane2 : GIPC2. Lane 3 : 293T. Lane 4 : HepG2. Lane 5 : HeLa.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of GIPC in HeLa cells, The cell was stained with GIPC2 monoclonal antibody, clone AT1G10 (Cat # MAB11190) at 2-5 ug for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — GIPC2
Entrez GeneID
54810Protein Accession#
NP_060125.4Gene Name
GIPC2
Gene Alias
FLJ20075, SEMCAP-2, SEMCAP2
Gene Description
GIPC PDZ domain containing family, member 2
Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000038608|PDZ domain protein GIPC2|semaF cytoplasmic domain associated protein 2|semaphorin cytoplasmic domain associated protein 2
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Interactome
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Disease
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