CIB1 monoclonal antibody, clone 1D1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HT-29 cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: 293T cell lysate; Lane 2: CIB1 Transfected 293T cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CIB1 monoclonal antibody, clone 1D1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant CIB1.
Immunogen
Recombinant protein corresponding to full length human CIB1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HT-29 cell lysate.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: 293T cell lysate; Lane 2: CIB1 Transfected 293T cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CIB1 monoclonal antibody, clone 1D1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CIB1
Entrez GeneID
10519GeneBank Accession#
NM_006384Protein Accession#
NP_006375Gene Name
CIB1
Gene Alias
CIB, KIP, KIP1, SIP2-28
Gene Description
calcium and integrin binding 1 (calmyrin)
Omim ID
602293Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the calcium-binding protein family. The specific function of this protein has not yet been determined; however this protein is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining. This protein also interacts with integrin alpha(IIb)beta(3), which may implicate this protein as a regulatory molecule for alpha(IIb)beta(3). [provided by RefSeq
Other Designations
DNA-dependent protein kinase interacting protein|Snk interacting protein 2-28|calcium and integrin binding 1|calcium and integrin binding protein|calcium and integrin binding, protein kinase interacting protein|calmyrin
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Interactome
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Disease
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Publication Reference
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CIB1 is essential for mouse spermatogenesis.
Yuan W, Leisner TM, McFadden AW, Clark S, Hiller S, Maeda N, O'Brien DA, Parise LV.
Molecular and Cellular Biology 2006 Nov; 26(22):8507.
Application：WB, Mouse, MEFs, Testis.
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CIB1 is an endogenous inhibitor of agonist-induced integrin alphaIIbbeta3 activation.
Yuan W, Leisner TM, McFadden AW, Wang Z, Larson MK, Clark S, Boudignon-Proudhon C, Lam SC, Parise LV.
The Journal of Cell Biology 2006 Jan; 172(2):169.
Application：IF, IP, WB-Tr, Human, Megakaryocytes, Platelets, Recombinant protein.
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CIB1 is essential for mouse spermatogenesis.
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