NME1 monoclonal antibody, clone 4B2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) against NME1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissues using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833).
Immunofluorescence
Immunofluorescence analysis of HeLa cells using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NME1.
Immunogen
Recombinant protein corresponding to human NME1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) against NME1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissues using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833).Immunofluorescence
Immunofluorescence analysis of HeLa cells using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) (green) and negative control (purple). -
Gene Info — NME1
Entrez GeneID
4830Gene Name
NME1
Gene Alias
AWD, GAAD, NB, NBS, NDPK-A, NDPKA, NM23, NM23-H1
Gene Description
non-metastatic cells 1, protein (NM23A) expressed in
Gene Ontology
HyperlinkGene Summary
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
Other Designations
NDP kinase A|OTTHUMP00000174772
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Interactome
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Pathway
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Disease
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