MAP3K5 monoclonal antibody, clone 2E4
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) against human MAP3K5 (aa: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and prostate tissues (B) using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798).
Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green) and negative control (purple).
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MAP3K5.
Immunogen
Recombinant protein corresponding to human MAP3K5.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Recombinant protein)
Western blot analysis using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) against human MAP3K5 (aa: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and prostate tissues (B) using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798).Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green) and negative control (purple). -
Gene Info — MAP3K5
Entrez GeneID
4217Gene Name
MAP3K5
Gene Alias
ASK1, MAPKKK5, MEKK5
Gene Description
mitogen-activated protein kinase kinase kinase 5
Omim ID
602448Gene Ontology
HyperlinkGene Summary
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq
Other Designations
MAP/ERK kinase kinase 5|MAPK/ERK kinase kinase 5|OTTHUMP00000017275|apoptosis signal regulating kinase
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com