JAK3 monoclonal antibody, clone 5H2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) against Jurkat cell lysate.
Immunofluorescence
Confocal immunofluorescence analysis of HeLa (upper) and HepG2 (bottom) cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence
Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of HeLa cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant JAK3.
Immunogen
Recombinant protein corresponding to human JAK3.
Host
Mouse
Theoretical MW (kDa)
125
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) against Jurkat cell lysate.Immunofluorescence
Confocal immunofluorescence analysis of HeLa (upper) and HepG2 (bottom) cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Immunofluorescence
Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (blue) and negative control (red). -
Gene Info — JAK3
Entrez GeneID
3718Gene Name
JAK3
Gene Alias
JAK-3, JAK3_HUMAN, JAKL, L-JAK, LJAK
Gene Description
Janus kinase 3 (a protein tyrosine kinase, leukocyte)
Omim ID
600173Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq
Other Designations
Janus kinase 3|leukocyte Janus kinase|tyrosine-protein kinase JAK3
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Interactome
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Pathway
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Disease
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Publication Reference
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Novel compound heterozygous mutations in a Japanese girl with Janus kinase 3 deficiency.
Sato T, Okano T, Tanaka-Kubota M, Kimura S, Miyamoto S, Ono S, Yamashita M, Mitsuiki N, Takagi M, Imai K, Kajiwara M, Ebato T, Ogata S, Oda H, Ohara O, Kanegane H, Morio T.
Pediatrics International 2016 Sep; 58(10):1076.
Application：WB, Human, Lymphoblastoid cell line.
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Novel compound heterozygous mutations in a Japanese girl with Janus kinase 3 deficiency.
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