CTNNB1 monoclonal antibody, clone 4D5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) against CTNNB1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissues using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) .
Immunofluorescence
Immunofluorescence analysis of A-549 (upper) and SK-BR-3 (bottom) cells using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of A-549 cells using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CTNNB1.
Immunogen
Recombinant protein corresponding to human CTNNB1.
Host
Mouse
Theoretical MW (kDa)
85
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) against CTNNB1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissues using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) .Immunofluorescence
Immunofluorescence analysis of A-549 (upper) and SK-BR-3 (bottom) cells using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A-549 cells using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) (green) and negative control (purple). -
Gene Info — CTNNB1
Entrez GeneID
1499Gene Name
CTNNB1
Gene Alias
CTNNB, DKFZp686D02253, FLJ25606, FLJ37923
Gene Description
catenin (cadherin-associated protein), beta 1, 88kDa
Gene Ontology
HyperlinkGene Summary
Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM
Other Designations
OTTHUMP00000165222|OTTHUMP00000165223|catenin (cadherin-associated protein), beta 1 (88kD)|catenin beta-1
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Interactome
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Pathway
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Disease
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