CTNNB1 recombinant monoclonal antibody, clone R07-9D5
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More Files
- More Functions
Western Blot
Western blot analysis of beta Catenin in NTERA-2, 3T3 lysates using human beta Catenin recombinant monoclonal antibody, clone R07-9D5 (Cat # RAB01560).
Immunocytochemistry
Immunocytochemistry analysis of beta Catenin (green) in 293T using human beta Catenin recombinant monoclonal antibody, clone R07-9D5 (Cat # RAB01560), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human beta Catenin.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human beta Catenin
Theoretical MW (kDa)
Calculated MW: 85 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of beta Catenin in NTERA-2, 3T3 lysates using human beta Catenin recombinant monoclonal antibody, clone R07-9D5 (Cat # RAB01560).Immunocytochemistry
Immunocytochemistry analysis of beta Catenin (green) in 293T using human beta Catenin recombinant monoclonal antibody, clone R07-9D5 (Cat # RAB01560), and DAPI(blue). -
Gene Info — CTNNB1
Entrez GeneID
1499Protein Accession#
P35222Gene Name
CTNNB1
Gene Alias
CTNNB, DKFZp686D02253, FLJ25606, FLJ37923
Gene Description
catenin (cadherin-associated protein), beta 1, 88kDa
Gene Ontology
HyperlinkGene Summary
Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM
Other Designations
OTTHUMP00000165222|OTTHUMP00000165223|catenin (cadherin-associated protein), beta 1 (88kD)|catenin beta-1
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Interactome
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Pathway
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Disease
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