PTEN monoclonal antibody, clone 1B8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of PTEN monoclonal antibody, clone 1B8 (Cat # MAB10405) against HeLa (1) and NIH/3T3 (2) cell lysate.
Immunofluorescence
Confocal immunofluorescence analysis of HeLa (A) and HepG2 (B) cells using PTEN monoclonal antibody, clone 1B8 (Cat # MAB10405) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of HeLa cells using PTEN monoclonal antibody, clone 1B8 (Cat # MAB10405) (B) and negative control (A).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PTEN.
Immunogen
Recombinant protein corresponding to human PTEN.
Host
Mouse
Theoretical MW (kDa)
54
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PTEN monoclonal antibody, clone 1B8 (Cat # MAB10405) against HeLa (1) and NIH/3T3 (2) cell lysate.Immunofluorescence
Confocal immunofluorescence analysis of HeLa (A) and HepG2 (B) cells using PTEN monoclonal antibody, clone 1B8 (Cat # MAB10405) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using PTEN monoclonal antibody, clone 1B8 (Cat # MAB10405) (B) and negative control (A). -
Gene Info — PTEN
Entrez GeneID
5728Gene Name
PTEN
Gene Alias
10q23del, BZS, MGC11227, MHAM, MMAC1, PTEN1, TEP1
Gene Description
phosphatase and tensin homolog
Gene Ontology
HyperlinkGene Summary
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq
Other Designations
MMAC1 phosphatase and tensin homolog deleted on chromosome 10|OTTHUMP00000020032|mutated in multiple advanced cancers 1
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Interactome
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Pathway
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Disease
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