S100A10 monoclonal antibody, clone 4E7E10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of S100A10 monoclonal antobody, clone 4E7E10 (Cat # MAB10389) against MCF-7 (1), HepG2 (2) and HeLa (3).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human nerve and ganglion cells using S100A10 monoclonal antobody, clone 4E7E10 (Cat # MAB10389).
Immunofluorescence
Confocal immunofluorescence analysis of HeLa (A) and L-02 (B) cells using S100A10 monoclonal antobody, clone 4E7E10 (Cat # MAB10389) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant S100A10.
Immunogen
Recombinant protein corresponding to human S100A10.
Host
Mouse
Theoretical MW (kDa)
11
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of S100A10 monoclonal antobody, clone 4E7E10 (Cat # MAB10389) against MCF-7 (1), HepG2 (2) and HeLa (3).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human nerve and ganglion cells using S100A10 monoclonal antobody, clone 4E7E10 (Cat # MAB10389).Immunofluorescence
Confocal immunofluorescence analysis of HeLa (A) and L-02 (B) cells using S100A10 monoclonal antobody, clone 4E7E10 (Cat # MAB10389) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — S100A10
Entrez GeneID
6281Gene Name
S100A10
Gene Alias
42C, ANX2L, ANX2LG, CAL1L, CLP11, Ca[1], GP11, MGC111133, P11, p10
Gene Description
S100 calcium binding protein A10
Omim ID
114085Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis. [provided by RefSeq
Other Designations
OTTHUMP00000015269|OTTHUMP00000015270|S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11))|S100 calcium-binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11))|annexin II ligand, calpactin I, lig
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Interactome
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Disease
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