PPP2R4 monoclonal antibody, clone 4D9
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) against recombinant human PPP2R4 protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue (A) and lung cancer tissue (B) using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PPP2R4.
Immunogen
Recombinant protein corresponding to human PPP2R4.
Host
Mouse
Theoretical MW (kDa)
41
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) against recombinant human PPP2R4 protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue (A) and lung cancer tissue (B) using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) (green) and negative control (red). -
Gene Info — PPP2R4
Entrez GeneID
5524Gene Name
PPP2R4
Gene Alias
MGC2184, PP2A, PR53, PTPA
Gene Description
protein phosphatase 2A activator, regulatory subunit 4
Omim ID
600756Gene Ontology
HyperlinkGene Summary
Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000022333|PP2A phosphatase activator|PP2A, subunit B'|phosphotyrosyl phosphatase activator|protein phosphatase 2A, regulatory subunit B'|protein phosphatase 2A, regulatory subunit B' (PR 53)
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Interactome
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Disease
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