RCHY1 monoclonal antibody, clone 1H10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) against HeLa (1), A-549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of PC-12 cells using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant RCHY1.
Immunogen
Recombinant protein corresponding to human RCHY1.
Host
Mouse
Theoretical MW (kDa)
30, 60
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) against HeLa (1), A-549 (2), MCF-7 (3) and PC-12 (4) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of PC-12 cells using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) (blue) and negative control (red). -
Gene Info — RCHY1
Entrez GeneID
25898Gene Name
RCHY1
Gene Alias
ARNIP, CHIMP, DKFZp586C1620, PIRH2, PRO1996, RNF199, ZNF363, hARNIP
Gene Description
ring finger and CHY zinc finger domain containing 1
Omim ID
607680Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
CH-rich interacting match with PLAG1|androgen-receptor N-terminal-interacting protein|zinc finger protein 363
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Interactome
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Pathway
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