Products

Product Description

Whole cell lysate (denatured).

Tissue

Kidney

Host

Human

Preparation Method

Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly.

Lysis Buffer

1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).

Quality Control Testing

12.5% SDS-PAGE Stained with Coomassie Blue.

SDS-PAGE Gel

Recommend Usage

The lysate is ready to load on SDS-PAGE for Western blotting.

Storage Buffer

In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).

Storage Instruction

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.