GRAP2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-GRAP2 full length
Host
Human
Theoretical MW (kDa)
38
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GRAP2 antibody (H00009402-M01) by Western Blots.
SDS-PAGE Gel
GRAP2 transfected lysate
Western Blot
Lane 1: GRAP2 transfected lysate ( 38 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — GRAP2
Entrez GeneID
9402GeneBank Accession#
BC025692Protein Accession#
AAH25692Gene Name
GRAP2
Gene Alias
GADS, GRAP-2, GRB2L, GRBLG, GRID, GRPL, GrbX, Grf40, Mona, P38
Gene Description
GRB2-related adaptor protein 2
Omim ID
604518Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq
Other Designations
GRB-2-like protein|GRB2-related protein with insert domain|OTTHUMP00000028837|SH3-SH2-SH3 adaptor molecule|growth factor receptor-binding protein|growth factor receptor-bound protein 2-related adaptor protein 2|hematopoietic cell-associated adaptor protei
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Interactome
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Pathway
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Disease
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