PUS1 monoclonal antibody (M01), clone 3A3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PUS1 expression in transfected 293T cell line by PUS1 monoclonal antibody (M01), clone 3A3.
Lane 1: PUS1 transfected lysate(44.4 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PUS1 transfected lysate using anti-PUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PUS1 MaxPab rabbit polyclonal antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PUS1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (69.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PUS1.
Immunogen
PUS1 (AAH02901, 1 a.a. ~ 399 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAGNAEPPPAGAACPQDRRSCSGRAGGDRVWEDGEHPAKKLKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (82); Rat (86)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (69.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PUS1 expression in transfected 293T cell line by PUS1 monoclonal antibody (M01), clone 3A3.
Lane 1: PUS1 transfected lysate(44.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PUS1 transfected lysate using anti-PUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PUS1 MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PUS1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PUS1
Entrez GeneID
80324GeneBank Accession#
BC002901Protein Accession#
AAH02901Gene Name
PUS1
Gene Alias
MGC11268, MLASA
Gene Description
pseudouridylate synthase 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants
Other Designations
pseudouridine synthase 1
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