DDX54 monoclonal antibody (M01), clone 2H6
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More Files
- More Functions
Western Blot (Cell lysate)
DDX54 monoclonal antibody (M01), clone 2H6 Western Blot analysis of DDX54 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody (M01), clone 2H6.
Lane 1: DDX54 transfected lysate(98.6 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DDX54 is 0.03 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to DDX54 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.18 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant DDX54.
Immunogen
DDX54 (NP_076977, 778 a.a. ~ 881 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DDRDSDEEGASDRRGPERRGGKRDRGQGASRPHAPGTPAGRVRPELKTKQQILKQRRRAQKLHFLQRGGLKQLSARNRRRVQELQQGAFGRGARSKKGKMRKRM
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (84)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.18 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
DDX54 monoclonal antibody (M01), clone 2H6 Western Blot analysis of DDX54 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody (M01), clone 2H6.
Lane 1: DDX54 transfected lysate(98.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DDX54 is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to DDX54 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — DDX54
Entrez GeneID
79039GeneBank Accession#
NM_024072Protein Accession#
NP_076977Gene Name
DDX54
Gene Alias
DP97, MGC2835
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Omim ID
611665Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase|DEAD box helicase 97 KDa
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