LEPRE1 monoclonal antibody (M01), clone 3C7
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More Files
- More Functions
Western Blot (Cell lysate)
LEPRE1 monoclonal antibody (M01), clone 3C7 Western Blot analysis of LEPRE1 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody (M01), clone 3C7.
Lane 1: LEPRE1 transfected lysate(46 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LEPRE1 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant LEPRE1.
Immunogen
LEPRE1 (AAH15309, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DPRVREVMNQNLAYYAAMLGEEHTRSIGPRESAKEYRQRSLLEKELLFFAYDVFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (92)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
LEPRE1 monoclonal antibody (M01), clone 3C7 Western Blot analysis of LEPRE1 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody (M01), clone 3C7.
Lane 1: LEPRE1 transfected lysate(46 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LEPRE1 is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — LEPRE1
Entrez GeneID
64175GeneBank Accession#
BC015309Protein Accession#
AAH15309Gene Name
LEPRE1
Gene Alias
GROS1, MGC117314, P3H1
Gene Description
leucine proline-enriched proteoglycan (leprecan) 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Two alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000008736|growth suppressor 1|leprecan|leprecan 1|prolyl 3-hydroxylase 1
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Interactome
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Publication Reference
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Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.
Ohe R, Aung NY, Meng H, Kabasawa T, Suto A, Tamazawa N, Yang S, Kato T, Yamakawa M.
Leukemia and Lymphoma 2016 Jul; 57(7):1687.
Application：IHC-P, Human, Non-neoplastic lymphoid.
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Proteomic dissection of the VHL interactome.
Lai Y, Song M, Hakala K, Weintraub ST, Shiio Y.
Journal of Proteome Research 2011 Nov; 10(11):5175.
Application：WB-Tr, Human, HEK 293T cells.
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Severe osteogenesis imperfecta in cyclophilin B-deficient mice.
Choi JW, Sutor SL, Lindquist L, Evans GL, Madden BJ, Bergen HR 3rd, Hefferan TE, Yaszemski MJ, Bram RJ.
PLoS Genetics 2009 Dec; 5(12):e1000750.
Application：WB, Mouse, Mouse embryonic fibroblasts (MEFs) and skin fibroblasts.
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Prolyl 3-hydroxylase 1 deficiency causes a recessive metabolic bone disorder resembling lethal/severe osteogenesis imperfecta.
Cabral WA, Chang W, Barnes AM, Weis M, Scott MA, Leikin S, Makareeva E, Kuznetsova NV, Rosenbaum KN, Tifft CJ, Bulas DI, Kozma C, Smith PA, Eyre DR, Marini JC.
Nature Genetics 2007 Feb; 39(3):359.
Application：WB, Mouse, Mouse osteoblasts.
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Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.
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