HIF1AN monoclonal antibody (M01), clone 1D8
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More Files
- More Functions
Western Blot (Cell lysate)
HIF1AN monoclonal antibody (M01), clone 1D8 Western Blot analysis of HIF1AN expression in Jurkat ( Cat # L017V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of HIF1AN expression in transfected 293T cell line by HIF1AN monoclonal antibody (M01), clone 1D8.
Lane 1: HIF1AN transfected lysate (Predicted MW: 40.3 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of HIF1AN transfected lysate using anti-HIF1AN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HIF1AN monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HIF1AN is 0.1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to HIF1AN on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (64.13 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant HIF1AN.
Immunogen
HIF1AN (AAH07719.1, 1 a.a. ~ 349 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAATAAEAVASGSGEPREEAGALGPAWDESQLRSYSFPTRPIPRLSQSDPRAEELIENEEPVVLTDTNLVYPALKWDLEYLQENIGNGDFSVYSASTHKFLYYDEKKMANFQNFKPRSNREEMKFHEFVEKLQDIQQRGGEERLYLQQTLNDTVGRKIVMDFLGFNWNWINKQQGKRGWGQLTSNLLLIGMEGNVTPAHYDEQQNFFAQIKGYKRCILFPPDQFECLYPYPVHHPCDRQSQVDFDNPDYERFPNFQNVVGYETVVGPGDVLYIPMYWWHHIESLLNGGITITVNFWYKGAPTPKRIEYPLKAHQKVAIMRNIEKMLGEALGNPQEVGPLLNTMIKGRYN
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (64.13 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HIF1AN monoclonal antibody (M01), clone 1D8 Western Blot analysis of HIF1AN expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of HIF1AN expression in transfected 293T cell line by HIF1AN monoclonal antibody (M01), clone 1D8.
Lane 1: HIF1AN transfected lysate (Predicted MW: 40.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of HIF1AN transfected lysate using anti-HIF1AN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HIF1AN monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HIF1AN is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to HIF1AN on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — HIF1AN
Entrez GeneID
55662GeneBank Accession#
BC007719Protein Accession#
AAH07719.1Gene Name
HIF1AN
Gene Alias
DKFZp762F1811, FIH1, FLJ20615, FLJ22027
Gene Description
hypoxia inducible factor 1, alpha subunit inhibitor
Omim ID
606615Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000020284|Peptide-aspartate beta-dioxygenase|factor inhibiting HIF1|hypoxia-inducible factor 1, alpha subunit inhibitor
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Interactome
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Disease
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