ALF 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
ALF transfected lysate.
Western Blot
Lane 1: ALF transfected lysate ( 52.69 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ALF full-length
Host
Human
Theoretical MW (kDa)
52.69
Interspecies Antigen Sequence
Mouse (71); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ALF antibody (H00011036-B01) by Western Blots.
SDS-PAGE Gel
ALF transfected lysate.
Western Blot
Lane 1: ALF transfected lysate ( 52.69 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GTF2A1L
Entrez GeneID
11036GeneBank Accession#
NM_006872.2Protein Accession#
-Gene Name
GTF2A1L
Gene Alias
ALF, MGC26254
Gene Description
general transcription factor IIA, 1-like
Omim ID
605358Gene Ontology
HyperlinkGene Summary
The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
Other Designations
GTF2A1-like factor|TFIIA alpha/beta like factor|TFIIA large subunit isoform ALF|TFIIA-alpha/beta-like factor
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Interactome
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Pathway
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Disease
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