Products

Western Blot (Transfected lysate)

Western Blot analysis of PCDHGA8 expression in transfected 293T cell line by PCDHGA8 monoclonal antibody (M01), clone 1C11.

Lane 1: PCDHGA8 transfected lysate(101.48 KDa).
Lane 2: Non-transfected lysate.

Western Blot (Recombinant protein)

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged PCDHGA8 is approximately 0.3ng/ml as a capture antibody.

ELISA

RNAi Knockdown (Antibody validated)

Western blot analysis of PCDHGA8 over-expressed 293 cell line, cotransfected with PCDHGA8 Validated Chimera RNAi ( Cat # H00009708-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDHGA8 monoclonal antibody (M01), clone 1C11 (Cat # H00009708-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Entrez GeneID

9708

GeneBank Accession#

NM_032088

Protein Accession#

NP_114477

Gene Name

PCDHGA8

Gene Alias

KIAA0327, PCDH-GAMMA-A8

Gene Description

protocadherin gamma subfamily A, 8

Omim ID

606295

Gene Ontology

Hyperlink

Gene Summary

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq

Other Designations

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