ZW10 monoclonal antibody (M05), clone 2E5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5.
Lane 1: ZW10 transfected lysate(88.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ZW10 is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi ( Cat # H00009183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZW10 monoclonal antibody (M05), clone 2E5 (Cat # H00009183-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ZW10.
Immunogen
ZW10 (NP_004715, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
masfvtevla hsgrlekedl gtrisrltrr veeikgevcn miskkysefl psmqsaqgli tqvdklsedi dllksriese vrrdlhvstg eftdlkqqle
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (86); Rat (85)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5.
Lane 1: ZW10 transfected lysate(88.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ZW10 is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi ( Cat # H00009183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZW10 monoclonal antibody (M05), clone 2E5 (Cat # H00009183-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — ZW10
Entrez GeneID
9183GeneBank Accession#
NM_004724Protein Accession#
NP_004715Gene Name
ZW10
Gene Alias
HZW10, KNTC1AP, MGC149821
Gene Description
ZW10, kinetochore associated, homolog (Drosophila)
Omim ID
603954Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase. [provided by RefSeq
Other Designations
ZW10 homolog, centromere/kinetochore protein|centromere/kinetochore protein zw10|zeste white 10 homolog
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