NMI monoclonal antibody (M01), clone 9D8
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More Files
- More Functions
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NMI.
Immunogen
NMI (AAH01268, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMKFLSVETPENDSQLSNISCSFQVSSKVPYEI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (64); Rat (67)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NMI monoclonal antibody (M01), clone 9D8 Western Blot analysis of NMI expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody (M01), clone 9D8.
Lane 1: NMI transfected lysate(35.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NMI is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi ( Cat # H00009111-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody (M01), clone 9D8 (Cat # H00009111-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — NMI
Entrez GeneID
9111GeneBank Accession#
BC001268Protein Accession#
AAH01268Gene Name
NMI
Gene Alias
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Gene Description
N-myc (and STAT) interactor
Omim ID
603525Gene Ontology
HyperlinkGene Summary
NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq
Other Designations
N-myc and STAT interactor|N-myc interactor|N-myc-interactor
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Interactome
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Disease
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