RUVBL1 monoclonal antibody (M01), clone 3G4-1F8
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More Files
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Western Blot (Tissue lysate)
RUVBL1 monoclonal antibody (M01), clone 3G4-1F8. Western Blot analysis of RUVBL1 expression in human pancreas.
Western Blot (Transfected lysate)
Western Blot analysis of RUVBL1 expression in transfected 293T cell line by RUVBL1 monoclonal antibody (M01), clone 3G4-1F8.
Lane 1: RUVBL1 transfected lysate (Predicted MW: 50.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RUVBL1 is approximately 0.3ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MAP3K7 and RUVBL1. HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-RUVBL1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to RUVBL1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (75.9 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant RUVBL1.
Immunogen
RUVBL1 (AAH02993, 1 a.a. ~ 456 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEINKVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (75.9 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
RUVBL1 monoclonal antibody (M01), clone 3G4-1F8. Western Blot analysis of RUVBL1 expression in human pancreas.Western Blot (Transfected lysate)
Western Blot analysis of RUVBL1 expression in transfected 293T cell line by RUVBL1 monoclonal antibody (M01), clone 3G4-1F8.
Lane 1: RUVBL1 transfected lysate (Predicted MW: 50.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RUVBL1 is approximately 0.3ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MAP3K7 and RUVBL1. HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-RUVBL1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to RUVBL1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RUVBL1
Entrez GeneID
8607GeneBank Accession#
BC002993Protein Accession#
AAH02993Gene Name
RUVBL1
Gene Alias
ECP54, INO80H, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A
Gene Description
RuvB-like 1 (E. coli)
Omim ID
603449Gene Ontology
HyperlinkOther Designations
INO80 complex subunit H|RuvB (E coli homolog)-like 1|RuvB-like 1|TATA binding protein interacting protein 49 kDa
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Interactome
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Pathway
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Disease
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Publication Reference
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The Relationship between RUVBL1 (Pontin, TIP49, NMP238) and BCL6 in Benign and Malignant Human Lymphoid Tissues.
Baron BW, Baron RM, Baron JM.
Biochemistry and Biophysics Reports 2016 Jul; 6:1.
Application：WB, Human, HEK 293T cells.
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Autoantibodies to RuvBL1 and RuvBL2: A novel systemic sclerosis-related antibody associated with diffuse cutaneous and skeletal muscle involvement.
Kaji K, Fertig N, Medsger TA Jr, Satoh T, Hoshino K, Hamaguchi Y, Hasegawa M, Lucas M, Schnure A, Ogawa F, Sato S, Takehara K, Fujimoto M, Kuwana M.
Arthritis Care & Research 2014 Apr; 66(4):575.
Application：IF, WB-Ce, Human, HEp-2, K562 cells, Serum.
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The Relationship between RUVBL1 (Pontin, TIP49, NMP238) and BCL6 in Benign and Malignant Human Lymphoid Tissues.
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