CHAF1B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: CHAF1B transfected lysate ( 61.5 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
CHAF1B transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CHAF1B full-length
Host
Human
Theoretical MW (kDa)
61.6
Interspecies Antigen Sequence
Mouse (83); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CHAF1B antibody (H00008208-B01) by Western Blots.
Western Blot
Lane 1: CHAF1B transfected lysate ( 61.5 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
CHAF1B transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CHAF1B
Entrez GeneID
8208GeneBank Accession#
NM_005441Protein Accession#
NP_005432Gene Name
CHAF1B
Gene Alias
CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
Gene Description
chromatin assembly factor 1, subunit B (p60)
Omim ID
601245Gene Ontology
HyperlinkGene Summary
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq
Other Designations
CAF-1 subunit B|CAF-I 60 kDa subunit|M-phase phosphoprotein 7|chromatin assembly factor 1 subunit B|chromatin assembly factor I p60 subunit|human chromatin assembly factor-I p60 subunit
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