TEF 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TEF full-length
Host
Human
Theoretical MW (kDa)
33.2
Interspecies Antigen Sequence
Mouse (97); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TEF antibody (H00007008-B01) by Western Blots.
SDS-PAGE Gel
TEF transfected lysate.
Western Blot
Lane 1: TEF transfected lysate ( 33.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TEF
Entrez GeneID
7008GeneBank Accession#
NM_003216.2Protein Accession#
-Gene Name
TEF
Gene Alias
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Gene Description
thyrotrophic embryonic factor
Omim ID
188595Gene Ontology
HyperlinkGene Summary
Thyrotroph embryonic factor (TEF), a transcription factor, is a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
thyrotroph embryonic factor
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