STK10 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
STK10 transfected lysate.
Western Blot
Lane 1: STK10 transfected lysate ( 14.74 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-STK10 full-length
Host
Human
Theoretical MW (kDa)
14.74
Interspecies Antigen Sequence
Mouse (54); Rat (54)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-STK10 antibody (H00006793-B01) by Western Blots.
SDS-PAGE Gel
STK10 transfected lysate.
Western Blot
Lane 1: STK10 transfected lysate ( 14.74 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — STK10
Entrez GeneID
6793GeneBank Accession#
BC014413Protein Accession#
AAH14413Gene Name
STK10
Gene Alias
LOK, PRO2729
Gene Description
serine/threonine kinase 10
Omim ID
603919Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq
Other Designations
lymphocyte-oriented kinase
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Interactome
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Disease
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