SEC13 monoclonal antibody (M02), clone 1G7
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More Files
- More Functions
Immunoprecipitation
Immunoprecipitation of SEC13 transfected lysate using anti-SEC13 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SEC13 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SEC13 is 0.03 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to SEC13 on HeLa cell . [antibody concentration 40 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SEC13.
Immunogen
SEC13 (NP_109598, 226 a.a. ~ 324 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SIGLPTSTIASCSQDGRVFIWTCDDASSNTWSPKLLHKFNDVVWHVSWSITANILAVSGGDNKVTLWKESVDGQWVCISDVNKGQGSVSASVTEGQQNE
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Immunoprecipitation
Immunoprecipitation of SEC13 transfected lysate using anti-SEC13 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SEC13 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SEC13 is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SEC13 on HeLa cell . [antibody concentration 40 ug/ml] -
Gene Info — SEC13
Entrez GeneID
6396GeneBank Accession#
NM_030673Protein Accession#
NP_109598Gene Name
SEC13
Gene Alias
D3S1231E, SEC13L1, SEC13R, npp-20
Gene Description
SEC13 homolog (S. cerevisiae)
Omim ID
600152Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq
Other Designations
SEC13 protein|SEC13-like 1 isoform|SEC13-related protein
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Interactome
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Disease
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