PRSS8 monoclonal antibody (M11A), clone 3C4
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More Files
- More Functions
Western Blot (Cell lysate)
PRSS8 monoclonal antibody (M11A), clone 3C4. Western Blot analysis of PRSS8 expression in Raw 264.7.
Western Blot (Cell lysate)
PRSS8 monoclonal antibody (M11A), clone 3C4. Western Blot analysis of PRSS8 expression in HepG2.
Western Blot (Cell lysate)
PRSS8 monoclonal antibody (M11A), clone 3C4. Western Blot analysis of PRSS8 expression in PC-12.
Western Blot (Cell lysate)
PRSS8 monoclonal antibody (M11A), clone 3C4. Western Blot analysis of PRSS8 expression in NIH/3T3.
Western Blot (Transfected lysate)
Western Blot analysis of PRSS8 expression in transfected 293T cell line by PRSS8 monoclonal antibody (M11A), clone 3C4.
Lane 1: PRSS8 transfected lysate (Predicted MW: 36.4 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PRSS8 transfected lysate using anti-PRSS8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRSS8 MaxPab rabbit polyclonal antibody.
Western Blot detection against Immunogen (63.47 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PRSS8.
Immunogen
PRSS8 (AAH01462, 1 a.a. ~ 343 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAQKGVLGPGQLGAVAILLYLGLLRSGTGAEGAEAPCGVAPQARITGGSSAVAGQWPWQVSITYEGVHVCGGSLVSEQWVLSAAHCFPSEHHKEAYEVKLGAHQLDSYSEDAKVSTLKDIIPHPSYLQEGSQGDIALLQLSRPITFSRYIRPICLPAANASFPNGLHCTVTGWGHVAPSVSLLTPKPLQQLEVPLISRETCNCLYNIDAKPEEPHFVQEDMVCAGYVEGGKDACQGDSGGPLSCPVEGLWYLTGIVSWGDACGARNRPGVYTLASSYASWIQSKVTELQPRVVPQTQESQPDSNLCGSHLAFSSAPAQGLLRPILFLPLGLALGLLSPWLSEH
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgM Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (63.47 KDa) .
Storage Buffer
In ascites fluid
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PRSS8 monoclonal antibody (M11A), clone 3C4. Western Blot analysis of PRSS8 expression in Raw 264.7.Western Blot (Cell lysate)
PRSS8 monoclonal antibody (M11A), clone 3C4. Western Blot analysis of PRSS8 expression in HepG2.Western Blot (Cell lysate)
PRSS8 monoclonal antibody (M11A), clone 3C4. Western Blot analysis of PRSS8 expression in PC-12.Western Blot (Cell lysate)
PRSS8 monoclonal antibody (M11A), clone 3C4. Western Blot analysis of PRSS8 expression in NIH/3T3.Western Blot (Transfected lysate)
Western Blot analysis of PRSS8 expression in transfected 293T cell line by PRSS8 monoclonal antibody (M11A), clone 3C4.
Lane 1: PRSS8 transfected lysate (Predicted MW: 36.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PRSS8 transfected lysate using anti-PRSS8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRSS8 MaxPab rabbit polyclonal antibody.ELISA
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Gene Info — PRSS8
Entrez GeneID
5652GeneBank Accession#
BC001462Protein Accession#
AAH01462Gene Name
PRSS8
Gene Alias
CAP1, PROSTASIN
Gene Description
protease, serine, 8
Omim ID
600823Gene Ontology
HyperlinkGene Summary
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine. [provided by RefSeq
Other Designations
channel-activating protease 1|prostasin
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Interactome
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Disease
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Publication Reference
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Prostate cancer-associated urinary proteomes differ before and after prostatectomy.
Yan Feng, Shengzhi Liu, Rongrong Zha, Xun Sun, Kexin Li, Di Wu, Uma K Aryal, Michael Koch, Bai-Yan Li, Hiroki Yokota.
Therapeutic Advances in Medical Oncology 2022 Oct; 14:1758835922.
Application：WB-Ce, Human, Mouse, Human urine, TRAMP cells.
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Prostate cancer-associated urinary proteomes differ before and after prostatectomy.
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