POLR2E (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of POLR2E transfected lysate using rabbit polyclonal anti-POLR2E and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-POLR2E.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99%); Rat (99%)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of POLR2E transfected lysate using rabbit polyclonal anti-POLR2E and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-POLR2E.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-POLR2E (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-POLR2E (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — POLR2E
Entrez GeneID
5434Gene Name
POLR2E
Gene Alias
RPABC1, RPB5, XAP4, hRPB25, hsRPB5
Gene Description
polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
Omim ID
180664Gene Ontology
HyperlinkGene Summary
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq
Other Designations
DNA directed RNA polymerase II 23 kda polypeptide|DNA directed RNA polymerase II polypeptide E|polymerase (RNA) II (DNA directed) polypeptide E (25kD)
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Interactome
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Pathway
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Disease
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