NEFH monoclonal antibody (M01), clone 2E8
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More Files
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Western Blot (Cell lysate)
NEFH monoclonal antibody (M01), clone 2E8 Western Blot analysis of NEFH expression in HeLa ( Cat # L013V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NEFH is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.85 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NEFH.
Immunogen
NEFH (NP_066554, 263 a.a. ~ 363 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (95)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.85 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NEFH monoclonal antibody (M01), clone 2E8 Western Blot analysis of NEFH expression in HeLa ( Cat # L013V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NEFH is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — NEFH
Entrez GeneID
4744GeneBank Accession#
NM_021076Protein Accession#
NP_066554Gene Name
NEFH
Gene Alias
NFH
Gene Description
neurofilament, heavy polypeptide
Gene Ontology
HyperlinkGene Summary
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq
Other Designations
neurofilament triplet H protein|neurofilament, heavy polypeptide 200kDa
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Interactome
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Pathway
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Disease
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