NCL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NCL transfected lysate.
Western Blot
Lane 1: NCL transfected lysate ( 76.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NCL full-length
Host
Human
Theoretical MW (kDa)
76.6
Interspecies Antigen Sequence
Mouse (78); Rat (80)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NCL antibody (H00004691-D01P) by Western Blots.
SDS-PAGE Gel
NCL transfected lysate.
Western Blot
Lane 1: NCL transfected lysate ( 76.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NCL
Entrez GeneID
4691GeneBank Accession#
NM_005381Protein Accession#
NP_005372.2Gene Name
NCL
Gene Alias
C23, FLJ45706
Gene Description
nucleolin
Omim ID
164035Gene Ontology
HyperlinkGene Summary
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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