MARCKS monoclonal antibody (M04), clone 2H4
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant MARCKS.
Immunogen
MARCKS (NP_002347, 2 a.a. ~ 65 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAESGAKEELQANGSAP
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (32.78 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MARCKS monoclonal antibody (M04), clone 2H4. Western Blot analysis of MARCKS expression in Raw 264.7 ( Cat # L024V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MARCKS is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — MARCKS
Entrez GeneID
4082GeneBank Accession#
NM_002356Protein Accession#
NP_002347Gene Name
MARCKS
Gene Alias
80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL
Gene Description
myristoylated alanine-rich protein kinase C substrate
Omim ID
177061Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000017045|myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)|phosphomyristin
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