ID2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ID2 transfected lysate
Western Blot
Lane 1: ID2 transfected lysate ( 14.85 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ID2 full-length
Host
Human
Theoretical MW (kDa)
14.85
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ID2 antibody (H00003398-B01) by Western Blots.
SDS-PAGE Gel
ID2 transfected lysate
Western Blot
Lane 1: ID2 transfected lysate ( 14.85 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ID2
Entrez GeneID
3398GeneBank Accession#
BC030639Protein Accession#
AAH30639Gene Name
ID2
Gene Alias
GIG8, ID2A, ID2H, MGC26389, bHLHb26
Gene Description
inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
Omim ID
600386Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene. [provided by RefSeq
Other Designations
DNA-binding protein inhibitor ID2|OTTHUMP00000140258|cell growth-inhibiting gene 8|helix-loop-helix protein ID2|inhibitor of DNA binding 2|inhibitor of differentiation 2
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Interactome
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Pathway
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Disease
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