ELK3 monoclonal antibody (M02), clone 3A12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ELK3 expression in transfected 293T cell line by ELK3 monoclonal antibody (M02), clone 3A12.
Lane 1: ELK3 transfected lysate(44.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ELK3 is approximately 0.3ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ELK3.
Immunogen
ELK3 (NP_005221.2, 101 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SRESLLLQDSDCKASPEGREAHKHGLAALRSTSRNEYIHSGLYSSFTINSLQNPPDAFKAIKTEKLEEPPEDSPPVEEVRTVIRFVTNKTDKHVTRPVVS
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ELK3 expression in transfected 293T cell line by ELK3 monoclonal antibody (M02), clone 3A12.
Lane 1: ELK3 transfected lysate(44.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ELK3 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — ELK3
Entrez GeneID
2004GeneBank Accession#
NM_005230Protein Accession#
NP_005221.2Gene Name
ELK3
Gene Alias
ERP, NET, SAP2
Gene Description
ELK3, ETS-domain protein (SRF accessory protein 2)
Omim ID
600247Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq
Other Designations
ELK3 protein|ETS-domain protein|SRF accessory protein 2
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Interactome
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Disease
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