EIF2S1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
EIF2S1 transfected lysate.
Western Blot
Lane 1: EIF2S1 transfected lysate ( 36.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EIF2S1 full-length
Host
Human
Theoretical MW (kDa)
36.1
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EIF2S1 antibody (H00001965-D01P) by Western Blots.
SDS-PAGE Gel
EIF2S1 transfected lysate.
Western Blot
Lane 1: EIF2S1 transfected lysate ( 36.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EIF2S1
Entrez GeneID
1965GeneBank Accession#
NM_004094Protein Accession#
NP_004085.1Gene Name
EIF2S1
Gene Alias
EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Gene Description
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Omim ID
603907Gene Ontology
HyperlinkGene Summary
The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM
Other Designations
eIF-2-alpha|eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD )
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