DPH2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DPH2 transfected lysate.
Western Blot
Lane 1: DPH2 transfected lysate ( 53.9 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DPH2 full-length
Host
Human
Theoretical MW (kDa)
53.9
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DPH2 antibody (H00001802-B01) by Western Blots.
SDS-PAGE Gel
DPH2 transfected lysate.
Western Blot
Lane 1: DPH2 transfected lysate ( 53.9 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DPH2
Entrez GeneID
1802GeneBank Accession#
NM_001384.4Protein Accession#
NP_001375.2Gene Name
DPH2
Gene Alias
DPH2L2
Gene Description
DPH2 homolog (S. cerevisiae)
Omim ID
603456Gene Ontology
HyperlinkGene Summary
This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
DPH2-like 2|OTTHUMP00000010007|diphthamide biosynthesis protein 2|diphthamide biosynthesis-like protein 2|diptheria toxin resistance protein required for diphthamide biosynthesis-like 2
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Interactome
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Disease
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