CYB5A monoclonal antibody (M06), clone 1A8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant CYB5A.
Immunogen
CYB5A (AAH15182, 1 a.a. ~ 134 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (40.48 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody (M06), clone 1A8.
Lane 1: CYB5A transfected lysate(15.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CYB5A on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — CYB5A
Entrez GeneID
1528GeneBank Accession#
BC015182Protein Accession#
AAH15182Gene Name
CYB5A
Gene Alias
CYB5, MCB5
Gene Description
cytochrome b5 type A (microsomal)
Omim ID
250790Gene Ontology
HyperlinkOther Designations
cytochrome b-5|cytochrome b5 (microsomal)|microsomal cytochrome b5|type 1 cyt-b5
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Interactome
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Publication Reference
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Pomegranate extracts impact the androgen biosynthesis pathways in prostate cancer models in vitro and in vivo.
Ming DS, Pham S, Deb S, Chin MY, Kharmate G, Adomat H, Beheshti EH, Locke J, Guns ET.
The Journal of Steroid Biochemistry and Molecular Biology 2014 Sep; 143:19.
Application:WB-Ce, Human, LNCaP, 22RV1 cells.
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Pomegranate extracts impact the androgen biosynthesis pathways in prostate cancer models in vitro and in vivo.
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