CYB5A monoclonal antibody (M05), clone 4C2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant CYB5A.
Immunogen
CYB5A (AAH15182, 1 a.a. ~ 134 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (40.48 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CYB5A on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CYB5A MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CYB5A is 3 ng/ml as a capture antibody.ELISA
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Gene Info — CYB5A
Entrez GeneID
1528GeneBank Accession#
BC015182Protein Accession#
AAH15182Gene Name
CYB5A
Gene Alias
CYB5, MCB5
Gene Description
cytochrome b5 type A (microsomal)
Omim ID
250790Gene Ontology
HyperlinkOther Designations
cytochrome b-5|cytochrome b5 (microsomal)|microsomal cytochrome b5|type 1 cyt-b5
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Interactome
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Publication Reference
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Cholesterol deprivation drives DHEA biosynthesis in human adrenals.
Emanuele Pignatti, Emre Murat Altinkilic, Konstantin Bräutigam, Michael Grössl, Aurel Perren, Mihaela Zavolan, Christa E Flück.
Endocrinology 2022 Jul; 163(7):bqac076.
Application:IF, Human, Human adrenal.
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Cholesterol deprivation drives DHEA biosynthesis in human adrenals.
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