CRK & SHC1 Protein Protein Interaction Antibody Pair
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More Files
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In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between CRK and SHC1. Mahlavu cells were stained with anti-CRK rabbit purified polyclonal antibody 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Representative image of Proximity Ligation Assay of protein-protein interactions between CRK and SHC1. HeLa cells were stained with anti-CRK rabbit purified polyclonal antibody 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the CRK protein, and the other against the SHC1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between CRK and SHC1. HeLa cells were stained with anti-CRK rabbit purified polyclonal antibody 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. CRK rabbit purified polyclonal antibody (100 ug)
2. SHC1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between CRK and SHC1. Mahlavu cells were stained with anti-CRK rabbit purified polyclonal antibody 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — CRK
Entrez GeneID
1398Gene Name
CRK
Gene Alias
CRKII
Gene Description
v-crk sarcoma virus CT10 oncogene homolog (avian)
Omim ID
164762Gene Ontology
HyperlinkGene Summary
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq
Other Designations
avian sarcoma virus CT10 (v-crk) oncogene homolog|v-crk avian sarcoma virus CT10 oncogene homolog|v-crk sarcoma virus CT10 oncogene homolog
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Gene Info — SHC1
Entrez GeneID
6464Gene Name
SHC1
Gene Alias
FLJ26504, SHC, SHCA
Gene Description
SHC (Src homology 2 domain containing) transforming protein 1
Omim ID
600560Gene Ontology
HyperlinkOther Designations
OTTHUMP00000035409|OTTHUMP00000035471|SHC (Src homology 2 domain-containing) transforming protein 1|SHC-transforming protein 1
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Interactome
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Pathway
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Disease
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