PDGFRB & CRKL Protein Protein Interaction Antibody Pair
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In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. HT-29 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. PC-3 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. A-549 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. A-549 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. HT-29 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. PC-3 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Representative image of Proximity Ligation Analysis of protein-protein interactions between PDGFRB and CRKL. HeLa cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the PDGFRB protein, and the other against the CRKL protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Analysis of protein-protein interactions between PDGFRB and CRKL. HeLa cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. PDGFRB rabbit purified polyclonal antibody (100 ug)
2. CRKL mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. HT-29 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. PC-3 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. A-549 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. A-549 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. HT-29 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. PC-3 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — CRKL
Entrez GeneID
1399Gene Name
CRKL
Gene Alias
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Gene Description
v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Omim ID
602007Gene Ontology
HyperlinkGene Summary
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic
Other Designations
v-crk avian sarcoma virus CT10 oncogene homolog-like
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Gene Info — PDGFRB
Entrez GeneID
5159Gene Name
PDGFRB
Gene Alias
CD140B, JTK12, PDGF-R-beta, PDGFR, PDGFR1
Gene Description
platelet-derived growth factor receptor, beta polypeptide
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq
Other Designations
beta platelet-derived growth factor receptor|platelet-derived growth factor receptor beta|soluble PDGFRb variant 1
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Interactome
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Pathway
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Disease
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