FLT1 & CRKL Protein Protein Interaction Antibody Pair
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In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. HT-29 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. PC-3 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. A-549 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. A-549 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. HT-29 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. PC-3 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Representative image of Proximity Ligation Analysis of protein-protein interactions between FLT1 and CRKL. HeLa cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the FLT1 protein, and the other against the CRKL protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Analysis of protein-protein interactions between FLT1 and CRKL. HeLa cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. FLT1 rabbit purified polyclonal antibody (100 ug)
2. CRKL mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. HT-29 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. PC-3 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. A-549 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. A-549 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. HT-29 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between FLT1 and CRKL. PC-3 cells were stained with anti-FLT1 rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — CRKL
Entrez GeneID
1399Gene Name
CRKL
Gene Alias
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Gene Description
v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Omim ID
602007Gene Ontology
HyperlinkGene Summary
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic
Other Designations
v-crk avian sarcoma virus CT10 oncogene homolog-like
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Gene Info — FLT1
Entrez GeneID
2321Gene Name
FLT1
Gene Alias
FLT, VEGFR1
Gene Description
fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
Omim ID
165070Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia
Other Designations
fms-related tyrosine kinase 1|soluble VEGF receptor 1-14|soluble VEGFR1 variant 2|soluble VEGFR1 variant 21|vascular endothelial growth factor/vascular permeability factor receptor
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Interactome
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Pathway
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Disease
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