CAPN1 & F2R Protein Protein Interaction Antibody Pair
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More Files
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Representative image of Proximity Ligation Assay of protein-protein interactions between CAPN1 and F2R. HeLa cells were stained with anti-CAPN1 rabbit purified polyclonal antibody 1:1200 and anti-F2R mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the CAPN1 protein, and the other against the F2R protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between CAPN1 and F2R. HeLa cells were stained with anti-CAPN1 rabbit purified polyclonal antibody 1:1200 and anti-F2R mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. CAPN1 rabbit purified polyclonal antibody (100 ug)
2. F2R mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — CAPN1
Entrez GeneID
823Gene Name
CAPN1
Gene Alias
CANP, CANP1, CANPL1, muCANP, muCL
Gene Description
calpain 1, (mu/I) large subunit
Omim ID
114220Gene Ontology
HyperlinkGene Summary
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq
Other Designations
calcium-activated neutral proteinase|calpain 1, large subunit|calpain, large polypeptide L1|cell proliferation-inducing protein 30
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Gene Info — F2R
Entrez GeneID
2149Gene Name
F2R
Gene Alias
CF2R, HTR, PAR1, TR
Gene Description
coagulation factor II (thrombin) receptor
Omim ID
187930Gene Ontology
HyperlinkGene Summary
Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq
Other Designations
coagulation factor II receptor|protease-activated receptor 1|thrombin receptor
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Interactome
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Pathway
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Disease
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