Rabbit Anti-Pig IgG (H&L) secondary antibody (Fluorescein)
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Specification
Product Description
Rabbit anti-pig IgG recognizes pig IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Pig IgG whole molecule
Host
Rabbit
Reactivity
Pig
Form
Lyophilized
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
2.4 moles DTAF per mole of rabbit IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
Lyophilized from 5 mM sodium phosphate, 70 mM NaCl, 50% (v/v) glycerol, pH 7.4
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.
D Blakeslee, M G Baines.
Journal of Immunological Methods 1976 Dec; 13(3-4):305.
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Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.
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