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Last updated: 2014/4/14
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BIOtech
BULK-new

AKT2 (phospho S474) polyclonal antibody

  • Catalog # : PAB0404
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT2.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S474 of human AKT2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • ELISA (1:1000)
    Dot Blot (1:100-500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of AKT2 (phospho S474) polyclonal antibody (Cat # PAB0404) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 ug/mL. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 208
  • Protein Accession#:
  • NP_001617;P31751
  • Gene Name:
  • AKT2
  • Gene Alias:
  • PKBB,PKBBETA,PRKBB,RAC-BETA
  • Gene Description:
  • v-akt murine thymoma viral oncogene homolog 2
  • Gene Summary:
  • This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
  • Other Designations:
  • Murine thymoma viral (v-akt) homolog-2,rac protein kinase beta
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