AKT2 monoclonal antibody, clone 148CT5.4.1.4
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Specification
Product Description
Mouse monoclonal antibody raised against human AKT2.
Immunogen
Recombinant protein corresponding to human AKT2.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:10-1:50)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysates reacted with AKT2 monoclonal antibody (Cat # MAB12302) at 1:100-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human prostate carcinoma reacted with AKT2 monoclonal antibody (Cat # MAB12302) at 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of Hela cell reacted with AKT2 monoclonal antibody (Cat # MAB12302) at 1:10-1:50 dilution. -
Gene Info — AKT2
Entrez GeneID
208Gene Name
AKT2
Gene Alias
PKBB, PKBBETA, PRKBB, RAC-BETA
Gene Description
v-akt murine thymoma viral oncogene homolog 2
Gene Ontology
HyperlinkGene Summary
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
Other Designations
Murine thymoma viral (v-akt) homolog-2|rac protein kinase beta
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Interactome
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Pathway
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Disease
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